Breaking Report: Plasmid Toolset for in vivo Expression or RNAi

On my desk to read today? A report of a set of plasmid vectors useful for in vivo expression of various forms of tagged or untagged genes or RNAi constructs.

Wang JW, Beck ES, McCabe BD. A modular toolset for recombination transgenesis and neurogenetic analysis of Drosophila. PLoS One. 2012;7(7):e42102. Epub 2012 Jul 25. PubMed PMID: 22848718

The authors indicate that the plasmids are available at AddGene.

Related resource: pVALIUM and pWALIUM vectors from the Trangenic RNAi Project (TRiP) at Harvard Med School.

Batch Search of TRiP Tools

Sneak peek for blog readers: here's a link to a batch search tool for TRiP stocks. Feedback welcome. It will be linked to from TRiP web pages on in vivo fly RNAi resources soon. Upload files should be .txt with one gene symbol (or other identifier) per line.

Breaking Report from the Transgenic RNAi Project (TRiP)

On my desk today? A breaking report from our lab space colleagues with the Transgenic RNAi Project (TRiP) here at Harvard Med School.

Ni et al. (2011) A genome-scale shRNA resource for transgenic RNAi in Drosophila. Nature Methods (e-published today ahead of print). PMID: 21460824.

Nominate Genes for TRiP Stock Production

The Trangenic RNAi Project (TRiP) is still taking community nominations for genes to go into the production pipeline for in vivo TRiP RNAi stocks. Nominate!

See the bottom of this page for the contact email for nomination:

http://www.flyrnai.org/TRiP-ACC-OVR.html

Breaking Report: Fly RNAi Methods

On my desk today?

Haley et al. (2010) Vectors and parameters that enhance the efficacy of RNAi-mediated gene disruption in transgenic Drosophila. PNAS. PMID: 20534445.

Bloomington Resources Paper

What's on my desk to read today? A new paper from the Bloomington Drosophila Stock Center (BDSC) that includes information about resources from the TRiP (in vivo RNAi) that are available at the BDSC:

Cook et al. (2010) New research resources at the Bloomington Drosophila Stock Center. Fly. 4(1):1-4.

Breaking Report: Transgenic RNAi Flies for Neurogenetics

What's on my desk today? A report from our sister group, the Transgenic RNAi Project (TRiP), which has been funded by HHMI and NIH to produce a large-scale collection of flies for in vivo RNAi.

Ni et al. (2009) A Drosophila Resource of Transgenic RNAi Lines for Neurogenetics. Genetics. PubMed ID 19487563.

Related Links:

TRiP web pages

TRiP toolbox at Bloomington

TRiP flies at Bloomington

VDRC

TRiP at Bloomington

Rumor has it the Transgenic RNAi Project (TRiP) "toolbox" stocks are a popular request at the Bloomington stock center now that they are available there (along with an increasing number of TRiP stocks with hairpins directed against specific genes).

RNAi in Whole Animals

The DRSC was originally focused on screening in cells. And we keep going down that path.

But we're moving in a new direction as well. Thanks to the Transgenic RNAi Project (TRiP). 

Shown here, the TRiP fly-pushing stations, which are being used for stock maintenance, making homozygous transgenic lines and more. 

Visit the TRiP site to view a list of available lines. 

More and more of these will be available at Bloomington soon (some are there already). In the meantime they can be requested from the TRiP directly. You can also read on-line about the TRiP strategy and plans, community nominations for genes TRiP should target, and screening the TRiP lines at the DRSC. 

Related links:

Bloomington Drosophila Stock Center

Vienna Drosophila RNAi Center