Breaking Report: Cross-Species Transgenesis

Also on my desk today--a pair of papers on techniques relevant to cross-species RNAi rescue.

Ejsmont et al. (2011) Recombination-mediated genetic engineering of large genomic DNA transgenes. Methods Mol Biol. 772:445-58. PubMed ID: 22065454.

Ejsmont et al. (2011) Production of fosmid genomic libraries optimized for liquid culture recombineering and cross-species transgenesis. Methods Mol Biol. 772:423-43. PubMed ID: 22065453.

Breaking Report: Ecdysone Receptor Co-Factors

Also on my desk today? Catching up on a new small-scale fly cell RNAi screen. The authors tested one dsRNA per gene with several different cell lines in this focused study.

Davis et al. (2011) Identification of common and cell type specific LXXLL motif EcR cofactors using a bioinformatics refined candidate RNAi screen in Drosophila melanogaster cell lines. BMC Dev Biol. 11(1):66. PubMed ID: 22050674.

Reminder: The DRSC can help with small-scale screens by building custom libraries in 96- or 384-well plates based on our existing libraries of templates.

Breaking Report: Stable Cell Lines

On my desk today? A new report on making stable cell lines.

Gonzalez et al. (2011) Generation of stable Drosophila cell lines using multicistronic vectors. In Nature's Scientific Reports. DOI: 10.1038/srep00075

DRSC Database Paper

Our database update paper is published. As always, feedback on the database and website is welcome. I'm hoping to catch up with some breaking research reports soon.

Flockhart et al. (2011) FlyRNAi.org--the database of the Drosophila RNAi screening center: 2012 update. Nucleic Acids Res. PubMed ID: 22067456.

Breaking Report: The ERK Signal Transduction Pathway

On my desk today? A paper that includes data from two screens performed at the DRSC. The screen datasets are available on our website--one way to access them is via the Experimental Data links following the listing for the paper on this page.

Friedman et al. (2011) Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. Sci Signal. 4(196):rs10. PubMed: 22028469.

Breaking Report: Rab5 & Chromosome Alignment

On my desk to ready today? A report on Rab5 GTPase and chromosome alignment. I confess that what drew me to the paper is technique, as the report includes live-cell imaging following RNAi, as well as simultaneous knockdown of two genes via RNAi.

Capalbo et al. (2011) Rab5 GTPase controls chromosome alignment through Lamin disassembly and relocation of the NuMA-like protein Mud to the poles during mitosis. Proc Natl Acad Sci U S A. PMID: 21987826.

Breaking Report: Review of Host-Pathogen Interaction Interactions

On my desk to read today? A review of work in the field of host-pathogen interactions. Studies at the DRSC and elsewhere using Drosophila cell-based RNAi have had an impact in the field.

Bier and Guichard (2011) Deconstructing host-pathogen interactions in Drosophila.
Dis Model Mech. PMID: 21979942.