Parsons LM, Grzeschik NA, Amaratunga K, Burke P, Quinn LM, Richardson HE. A Kinome RNAi Screen in Drosophila Identifies Novel Genes Interacting with Lgl, aPKC and Crb Cell Polarity Genes in Epithelial Tissues. G3 (Bethesda). 2017 Jun 13. PMID: 28611255.
From the abstract: "... To gain insight into the molecular mechanisms that coordinate cell polarity with tissue growth, we screened a boutique collection of RNAi stocks targeting the kinome for their capacity to modify Drosophila 'cell polarity' eye and wing phenotypes. ... We next conducted a secondary screen to test whether these cell polarity modifiers altered tissue overgrowth associated with depletion of Lgl in the wing. These screens identified Hippo, JNK, and Notch signalling pathways, previously linked to cell polarity regulation of tissue growth. Furthermore, novel pathways, not previously connected to cell polarity regulation of tissue growth were identified, including Wingless (Wg/Wnt), Ras and lipid/Phospho-inositol-3-kinase (PI3K) signalling pathways. Additionally, we demonstrated that the 'nutrient sensing' kinases, Salt Inducible Kinase 2 and 3 (SIK2 and 3) are potent modifiers of cell polarity phenotypes and regulators of tissue growth. ..."
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