Review--RNAi screening and signaling networks

Evans L, Sailem H, Vargas PP, Bakal C. Inferring signalling networks from images. J Microsc. 2013 Oct;252(1):1-7. PMID: 23841886.

From the abstract:  "This review describes the methodology used to map signalling networks using data generated in the context of RNAi screens."

Morphological complexity. Large-scale fly cell RNAi screen. Breaking report.

Data corresponding to the screen reported in this paper are available at flyrnai.org and through PubChem. The fly cell screen was done in Kc cells. The study includes mammalian cell follow-up.

Yin et al. A screen for morphological complexity identifies regulators of switch-like transitions between discrete cell shapes. Nature Cell Biology (2013) doi:10.1038/ncb2764.

*See also* Olson MF. Finding the shape-shifter genes. Nat Cell Biol. 2013 PMID: 23817235.

Differential RNAi and small libraries. Recent report.

Here's an example of a study that used two of the DRSC small libraries. These libraries have an optimal layout (limits edge effects) and average 2 or more unique dsRNAs per gene.

Garcia MA, Alvarez MS, Sailem H, Bousgouni V, Sero J, Bakal C. Differential RNAi screening provides insights into the rewiring of signalling networks during oxidative stress. Mol Biosyst. 2012 Oct;8(10):2605-13. PMID: 22790786.

Breaking Report: New Review

On my desk to read this morning? A new review from C. Bakal that includes discussion of complex image-based readouts as well as other topics in fly RNAi screening.

Bakal C. (2011) Drosophila RNAi screening in a postgenomic world. Brief Funct. Genomics. PubMed PMID: 21752787.