CRISPR knock-in approaches in Drosophila.

Scott J. Gratz, Fiona P. Ukken, C. Dustin Rubinstein, Gene Thiede, Laura K. Donohue, Alexander M. Cummings, and Kate M. O'Connor-Giles. Highly Specific and Efficient CRISPR/Cas9-Catalyzed Homology-Directed Repair in Drosophila. Genetics genetics.113.160713; early online January 29, 2014

More new reports on fly genome engineering.

Sebo ZL, Lee HB, Peng Y, Guo Y. A simplified and efficient germline-specific CRISPR/Cas9 system for Drosophila genomic engineering. Fly (Austin). 2013 Oct 18;8(1). PMID: 24141137.

Gratz SJ, Wildonger J, Harrison MM, O'Connor-Giles KM. CRISPR/Cas9-mediated genome engineering and the promise of designer flies on demand. Fly (Austin). 2013 Oct 2;7(4). PMID: 24088745.

Baena-Lopez LA, Alexandre C, Mitchell A, Pasakarnis L, Vincent JP. Accelerated homologous recombination and subsequent genome modification in Drosophila. Development. 2013 Oct 23. PMID: 24154526.

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CRISPR technology in flies

Of course this is not RNAi but it's the kind of technology we like to keep aware of here at the DRSC. So I thought I'd share it on the blog.

Gratz SJ, Cummings AM, Nguyen JN, Hamm DC, Donohue LK, Harrison MM, Wildonger J, O'Connor-Giles KM. Genome engineering of Drosophila with the CRISPR RNA-guided Cas9 nuclease. Genetics. 2013 May 24. PMID: 23709638.