Methods review on genome engineering in flies--Updated 2-3-2014

Beumer KJ and Carroll D. Targeted genome engineering techniques in Drosophila. Methods. 2014 Jan 8. pii: S1046-2023(13)00455-6. PMID: 24412316.

2-3-2014 update: Another review on fly genome engineering approaches.

Bassett AR, Liu JL. CRISPR/Cas9 and Genome Editing in Drosophila. J Genet Genomics. 2014 Jan 20;41(1):7-19. PMID: 24480743.

To view all posts tagged "CRISPRs" click here.

CRISPR/Cas9 in Drosophila cultured cells

RNAi screening in customized Drosophila cell lines has seemed like an inevitability since the emergence of the new genome engineering approaches.  With this report, that eventuality seems another step closer.

Bassett AR, Tibbit C, Ponting CP, Liu JL. Mutagenesis and homologous recombination in Drosophila cell lines using CRISPR/Cas9. Biol Open. 2013 Dec 10. PMID: 24326186.

CRISPRs in flies. Two breaking reports.

Bassett AR, Tibbit C, Ponting CP, Liu JL. Highly Efficient Targeted Mutagenesis of Drosophila with the CRISPR/Cas9 System. Cell Rep. 2013 Jun 27. PMID: 23827738.

Yu Z, Ren M, Wang Z, Zhang B, Rong YS, Jiao R, Gao G. Highly Efficient Genome Modifications Mediated by CRISPR/Cas9 in Drosophila. Genetics. 2013 Jul 5. PMID: 23833182.

See also this post on an earlier CRISPR paper.