Use of fly cells to study the cytoskeleton

Nye J, Buster DW, Rogers GC. The use of cultured Drosophila cells for studying the microtubule cytoskeleton. Methods Mol Biol. 2014;1136:81-101. PMID: 24633795.

From the abstract:  The authors "describe basic techniques for gene knockdown, transgene expression, preparation for fluorescence microscopy, and centrosome enrichment using cultured Drosophila cells with an emphasis on studying the microtubule cytoskeleton."

Breaking Report: The D17-c3 Cell Culture System

This paper describes use of D17-c3 cells. As the authors point out, a relatively small number of cell types are typical for RNAi screens but a large number of others are available from the DGRC.

Currie & Rogers (2011) Using the Drosophila melanogaster D17-c3 cell culture system to study cell motility. Nat Protoc 6(10):1632-41. PubMed ID: 21959242