A correspondence in Nature Methods raises concerns regarding in vivo RNAi fly stocks

Green et al. (2014) A Drosophila RNAi collection is subject to dominant phenotypic effects. Nature Methods 11, 222–223.

in vivo screen looks at role of RNA binding proteins in neuron morphogenesis

Olesnicky EC, Killian DJ, Garcia E, Morton MC, Rathjen AR, Sola IE, Gavis ER. Extensive Use of RNA Binding Proteins in Drosophila Sensory Neuron Dendrite Morphogenesis. G3 (Bethesda). 2013 Dec 20. pii: g3.113.009795v2. PMID: 24347626.

From the abstract:  "... we conducted an RNAi screen to identify Drosophila proteins annotated as either RNA binding proteins or translation factors that function in producing the complex dendritic trees of larval class IV dendritic arborization neurons. We identified 88 such proteins whose knock-down resulted in aberrant dendritic morphology ..."

in vivo RNAi screen related to fat storage

Baumbach J, Hummel P, Bickmeyer I, Kowalczyk KM, Frank M, Knorr K, Hildebrandt A, Riedel D, Jäckle H, Kühnlein RP. A Drosophila in vivo screen identifies store-operated calcium entry as a key regulator of adiposity. Cell Metab. 2014 PMID: 24506874.

New cell image analysis tool reported

Tsygankov D, Bilancia CG, Vitriol EA, Hahn KM, Peifer M, Elston TC. CellGeo: A computational platform for the analysis of shape changes in cells with complex geometries. J Cell Biol. 2014 Feb 3;204(3):443-60. PMID: 24493591.

DKFZ's GenomeRNAi releases v.12

The DKFZ's GenomeRNAi team recently announced release of a version 12 with new data sets and features. Check it out!

CRISPR knock-in approaches in Drosophila.

Scott J. Gratz, Fiona P. Ukken, C. Dustin Rubinstein, Gene Thiede, Laura K. Donohue, Alexander M. Cummings, and Kate M. O'Connor-Giles. Highly Specific and Efficient CRISPR/Cas9-Catalyzed Homology-Directed Repair in Drosophila. Genetics genetics.113.160713; early online January 29, 2014

Methods review on genome engineering in flies--Updated 2-3-2014

Beumer KJ and Carroll D. Targeted genome engineering techniques in Drosophila. Methods. 2014 Jan 8. pii: S1046-2023(13)00455-6. PMID: 24412316.

2-3-2014 update: Another review on fly genome engineering approaches.

Bassett AR, Liu JL. CRISPR/Cas9 and Genome Editing in Drosophila. J Genet Genomics. 2014 Jan 20;41(1):7-19. PMID: 24480743.

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