Breaking Reports: Cell-Based Screens

Catching up on my reading today. On the list:

Asensio et al. (2010) RNAi screen identifies a role for adaptor protein AP-3 in sorting to the regulated secretory pathway. J Cell Biol. 2010 Dec 13;191(6):1173-87. Protocol Notes: Screened in S2 cells using a library of "7,216 sequences that target Drosophila genes conserved to mammals."

Grimberg et al. (2010) Basic-Leucine Zipper Protein Cnc-C is a Substrate and Transcriptional Regulator of the Drosophila 26S Proteasome. Mol Cell Biol. Dec 13. Epub ahead of print. Protocol Notes: Screened the DRSC Transcription Factor library.

Primary Cells

On my desk today? A report that uses RNAi and primary cells to study muscles.

Rui et al. (2010) Sarcomere formation occurs by the assembly of multiple latent protein complexes. PLoS Genet. 6(11):e1001208.

Nominate Genes for TRiP Stock Production

The Trangenic RNAi Project (TRiP) is still taking community nominations for genes to go into the production pipeline for in vivo TRiP RNAi stocks. Nominate!

See the bottom of this page for the contact email for nomination:

http://www.flyrnai.org/TRiP-ACC-OVR.html