Wednesday, January 16, 2013

RNAi rescue assay and snRNAs. Breaking report.

Chen J, Waltenspiel B, Warren WD, Wagner EJ. Functional Analysis of the Integrator Subunit 12 Identifies a Microdomain that Mediates Activation of the Drosophila Integrator Complex. J Biol Chem. 2013 Jan 3. PubMed PMID: 23288851.

Image analysis script for stress granules and processing bodies. Breaking report.

These authors describe a script developed to analyze a specific set of phenotypes in high-throughput image-based screens such as RNAi screens.

Perez-Pepe M, Slomiansky V, Loschi M, Luchelli L, Neme M, Thomas MG, Boccaccio GL. BUHO: A MATLAB Script for the Study of Stress Granules and Processing Bodies by High-Throughput Image Analysis. PLoS One. 2012;7(12):e51495. doi: 10.1371/journal.pone.0051495. PMID: 23284702; PMCID: PMC3527446.

RNAi and RNA analysis. Cell cycle study. Breaking report.

These authors follow RNAi screening for DNA content in S2 cells with transcriptional profiling to uncover transcriptional networks. Bonke M, Turunen M, Sokolova M, Vähärautio A, Kivioja T, Taipale M, Björklund M, Taipale J. Transcriptional networks controlling the cell cycle. G3 (Bethesda). 2013 Jan;3(1):75-90. doi: 10.1534/g3.112.004283. PubMed PMID: 23316440.

Monday, January 7, 2013

Targeted in vivo screen for Notch pathway regulators. Breaking report.

Zhang J, Liu M, Su Y, Du J, Zhu AJ. A Targeted In Vivo RNAi Screen Reveals Deubiquitinases as New Regulators of Notch Signaling. G3 (Bethesda). 2012 Dec;2(12):1563-75. doi: 10.1534/g3.112.003780. PMID: 23275879; PMCID: PMC3516478.

S2 cell and RNAi system for exploring phagocytosis of L. donovani

Peltan A, Briggs L, Matthews G, Sweeney ST, Smith DF. Identification of Drosophila Gene Products Required for Phagocytosis of Leishmania donovani. PLoS One. 2012;7(12):e51831. doi: 10.1371/journal.pone.0051831. PMID: 23272175; PMCID: PMC3521716.

From the abstract:  "Utilising the phagocytic capability of Drosophila S2 cells, together with available tools for modulating gene expression by RNAi, we have developed an experimental system in which to identify host proteins of this type on a genome-wide scale ... These observations suggest that this experimental approach has the potential to identify a large number of host effectors required for efficient parasite uptake and maintenance.

New insights into the "germline-specific RNAi mechanism known as the Piwi-interacting RNA (piRNA) pathway"

Gell S L, Reenan RA. Mutations to the piRNA Pathway Component aubergine Enhance Meiotic Drive of Segregation Distorter in Drosophila melanogaster. Genetics. PMID: 23267055.