Monday, November 30, 2009

Breaking Report: Metazoan Secretory Pathway

What's on my desk to read today (besides the post-holiday pileup of emails)? A recent report based on a screen performed at the DRSC: Wendler et al. (2009) A genome-wide RNA interference screen identifies two novel components of the metzoan secretory pathway. EMBO Journal. Protocols notes: Luciferase assay readout (stable S2 cell line with the reporter construct), DRSC genome-wide library, follow up in human cells with siRNAs.

Tuesday, November 24, 2009

Thursday, November 12, 2009

Web Wednesday (well, Thurs)--Pathway Projector

Just learned about Pathway Projector, which describes itself as a zoomable interface for systems biology. Kono et al. (2009) Pathway Projector: Web-Based Zoomable Pathway Browser Using KEGG Atlas and Google Maps API. PLoSOne (free on-line access).

Monday, November 9, 2009

Breaking Report: JNK & Immunity Screen

What's on my desk today?

A report from Bond & Foley (2009) A Quantitative RNAi Screen for JNK Modifiers Identifies Pvr as a Novel Regulator of Drosophila Immune Signaling. PLoS Pathogen (Open Access).

From their abstract, "Our data illuminate a poorly understood arm of a critical and evolutionarily conserved innate immune response." Protocol Notes: Screened S2 cells; library in part from OpenBio; "in-cell western" approach compared phospho-JNK to Actin levels; 15 and 60 minute timepoints.