Monday, November 9, 2009

Breaking Report: JNK & Immunity Screen

What's on my desk today?

A report from Bond & Foley (2009) A Quantitative RNAi Screen for JNK Modifiers Identifies Pvr as a Novel Regulator of Drosophila Immune Signaling. PLoS Pathogen (Open Access).

From their abstract, "Our data illuminate a poorly understood arm of a critical and evolutionarily conserved innate immune response." Protocol Notes: Screened S2 cells; library in part from OpenBio; "in-cell western" approach compared phospho-JNK to Actin levels; 15 and 60 minute timepoints.

No comments: