Targeting PDZ domain-containing proteins. Breaking report.

On my desk to read today? This paper describing the results of in vivo RNAi knockdown of most PDZ domain-containing proteins.

Aranjuez G, Kudlaty E, Longworth MS, McDonald JA. On the role of PDZ domain-encoding genes in Drosophila border cell migration. G3 (Bethesda). 2012 Nov;2(11):1379-91. doi: 10.1534/g3.112.004093. Epub 2012 Nov 1. PubMed PMID: 23173089.

Large-scale RNAi screen. Breaking report.

On my desk to ready today? This disease-relevant report in an open access journal.


Voßfeldt H, Butzlaff M, Prüßing K, Ní Chárthaigh RA, Karsten P, Lankes A, Hamm S, Simons M, Adryan B, Schulz JB, Voigt A. Large-scale screen for modifiers of ataxin-3-derived polyglutamine-induced toxicity in Drosophila. PLoS One. 2012;7(11):e47452. doi: 10.1371/journal.pone.0047452. PubMed PMID: 23139745; PubMed Central PMCID: PMC3489908.

shRNA design. Breaking report.

Gu S, Jin L, Zhang Y, Huang Y, Zhang F, Valdmanis PN, Kay MA. The Loop Position of shRNAs and Pre-miRNAs Is Critical for the Accuracy of Dicer Processing In Vivo. Cell. 2012 Nov 9;151(4):900-11. doi:10.1016/j.cell.2012.09.042. PubMed PMID: 23141545.

Endogenous targets of RNAi. Breaking report.

Bronkhorst AW, van Cleef KW, Vodovar N, Ince IA, Blanc H, Vlak JM, Saleh MC, van Rij RP. The DNA virus Invertebrate iridescent virus 6 is a target of the Drosophila RNAi machinery. Proc Natl Acad Sci U S A. 2012 Nov 14. PubMed PMID: 23151511.

Fly RNAi and Legionella. Recent book chapter.

De Jesús DA, O'Connor TJ, Isberg RR. Analysis of Legionella Infection Using RNAi in Drosophila Cells. Methods Mol Biol. 2013;954:251-64. doi: 10.1007/978-1-62703-161-5_15. PubMed PMID: 23150401.

Endogenous RNAi Functions. Breaking Report.

Yamanaka S, Mehta S, Reyes-Turcu FE, Zhuang F, Fuchs RT, Rong Y, Robb GB, Grewal SI. RNAi triggered by specialized machinery silences developmental genes and retrotransposons. Nature. 2012 Nov 14. doi: 10.1038/nature11716. PubMed PMID: 23151475.

Maternal-Gal4 shRNA system. Breaking report.

Staller MV, Yan D, Randklev S, Bragdon MD, Wunderlich ZB, Tao R, Perkins LA, Depace AH, Perrimon N. Depleting Gene Activities in Early Drosophila Embryos with the "maternal-Gal4 - shRNA" System. Genetics. 2012 Oct 26. PubMed PMID: 23105012.

Touch me! Breaking report.

On my desk to ready today? This paper describing an in vivo RNAi screen of ion channels with larval responses to touch as the output.

Tsubouchi A, Caldwell JC, Tracey WD. Dendritic Filopodia, Ripped Pocket, NOMPC, and NMDARs Contribute to the Sense of Touch in Drosophila Larvae. Curr Biol. 2012 Oct 23. doi:pii: S0960-9822(12)01083-4. 10.1016/j.cub.2012.09.019. PubMed PMID: 23103192.