What's on my desk to read today? Kategaya et al. (2009) Bili Inhibits Wnt/β-Catenin Signaling by Regulating the Recruitment of Axin to LRP6. Free full text from Plos One.
Protocol Notes: Screened Clone 8 Cells (described in DasGupta R, Kaykas A, Moon RT, Perrimon N. Functional genomic analysis of the Wnt-wingless signaling pathway. Science. 2005 May 6;308(5723):826-33). Factors in defining what hits to follow up on included a look at potential off-target effects and comparison to hits identified in previous screen (the authors report that Bili was a weak or non-hit in screens in the DRSC database, suggesting specificity to the Wnt pathway). Follow-up tests included over-expression of Bili in Drosophila S2R+ and Clone 8 cells; test of shRNA directed against Bili in Drosophila embryos; tests in Zebrafish (with morpholinos); and tests in human cells (with siRNAs).
[Drawing above, very rough interpretation of part of their Figure 2.]