Breaking Report: Metazoan Secretory Pathway

What's on my desk to read today (besides the post-holiday pileup of emails)? A recent report based on a screen performed at the DRSC: Wendler et al. (2009) A genome-wide RNA interference screen identifies two novel components of the metzoan secretory pathway. EMBO Journal. Protocols notes: Luciferase assay readout (stable S2 cell line with the reporter construct), DRSC genome-wide library, follow up in human cells with siRNAs.

Local? Imaging Workshops

Local to HMS? Check out upcoming workshops offered by the imaging center. Training on image analysis software and more.

Web Wednesday (well, Thurs)--Pathway Projector

Just learned about Pathway Projector, which describes itself as a zoomable interface for systems biology. Kono et al. (2009) Pathway Projector: Web-Based Zoomable Pathway Browser Using KEGG Atlas and Google Maps API. PLoSOne (free on-line access).

Breaking Report: JNK & Immunity Screen

What's on my desk today?

A report from Bond & Foley (2009) A Quantitative RNAi Screen for JNK Modifiers Identifies Pvr as a Novel Regulator of Drosophila Immune Signaling. PLoS Pathogen (Open Access).

From their abstract, "Our data illuminate a poorly understood arm of a critical and evolutionarily conserved innate immune response." Protocol Notes: Screened S2 cells; library in part from OpenBio; "in-cell western" approach compared phospho-JNK to Actin levels; 15 and 60 minute timepoints.