Breaking Report: The ERK Signal Transduction Pathway

On my desk today? A paper that includes data from two screens performed at the DRSC. The screen datasets are available on our website--one way to access them is via the Experimental Data links following the listing for the paper on this page.

Friedman et al. (2011) Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. Sci Signal. 4(196):rs10. PubMed: 22028469.

Breaking Report: Rab5 & Chromosome Alignment

On my desk to ready today? A report on Rab5 GTPase and chromosome alignment. I confess that what drew me to the paper is technique, as the report includes live-cell imaging following RNAi, as well as simultaneous knockdown of two genes via RNAi.

Capalbo et al. (2011) Rab5 GTPase controls chromosome alignment through Lamin disassembly and relocation of the NuMA-like protein Mud to the poles during mitosis. Proc Natl Acad Sci U S A. PMID: 21987826.

Breaking Report: Review of Host-Pathogen Interaction Interactions

On my desk to read today? A review of work in the field of host-pathogen interactions. Studies at the DRSC and elsewhere using Drosophila cell-based RNAi have had an impact in the field.

Bier and Guichard (2011) Deconstructing host-pathogen interactions in Drosophila.
Dis Model Mech. PMID: 21979942.

Plate-to-List, List-to-Plate Conversion Tool

If you have ever copy-and-pasted data displayed like a 96- or 384-well plate to convert it to a simple list, or vice versa, then I expect you'll appreciate our newest online tool, the Plate-to-List, List-to-Plate conversion tool. It's designed for use with spreadsheets. We're finding it handy for some of our in-house stuff and expect it will be convenient for others as well. Big thanks to Ian Flockhart for developing the tool and getting it launched on the web very quickly!

DIOPT-DIST Updated

Our DIOPT-DIST tool was recently updated. Data from about 200 new GWAS studies was added. More info about the tool in the DIOPT paper and please feel free to ask us if you have questions.

Breaking Report: Shedding Light on NFkB

And yet another breaking paper on my desk today ...

Valanne et al. (2011) Large-scale RNAi screens add both clarity and complexity to Drosophila NFkB signaling. Dev Comp Immunol. PubMed ID: 21930155

Breaking Report: Neddylation & Hedgehog

Another breaking report on my desk to read today:

Du et al. (2011) In vivo RNAi scren reveals neddylation genes as novel regulators of hedgehog signaling. PLoS One. 6(9):e24168. PubMed ID: 21931660

Breaking Report: Review of RNAi screening

Norbert and I have a new review out that discusses cell-based and in vivo RNAi in various species and emphasizes interaction of the RNAi screening field with other fields of study.

Mohr & Perrimon (2011) RNAi screening: new approaches, understandings, and organisms. Wiley Interdiscip Rev RNA. doi: 10.1002/wrna. PubMed ID: 21953743

Breaking Report: The D17-c3 Cell Culture System

This paper describes use of D17-c3 cells. As the authors point out, a relatively small number of cell types are typical for RNAi screens but a large number of others are available from the DGRC.

Currie & Rogers (2011) Using the Drosophila melanogaster D17-c3 cell culture system to study cell motility. Nat Protoc 6(10):1632-41. PubMed ID: 21959242

Breaking Report: Review--Fly as Disease Model

I think this review on using Drosophila as a model to study cardiac function has a very nice introduction to molecular genetic methods--good reading for someone new to fly research.

Wolf & Rockman (2011) Drosophila, genetic screens, and cardiac function. Circ Res 109(7):794-806. PubMed ID: 21921272

Catching up

I'm back in town after a couple of weeks off. Prepare for a flurry of posts on breaking reports! And as always, if I've missed something, please feel free to email or comment to let me know.