My co-authors and I are pleased to present this Genetics journal "toolbox" series review article.
Mohr SE, Hu Y, Kim K, Housden BE, Perrimon N. Resources for Functional
Genomics Studies in Drosophila melanogaster. Genetics. PubMed PMID: 24653003.
Please note that the final published version is now available--complete with live links to the many websites and online software tools we refer to.
Showing posts with label Mohr. Show all posts
Showing posts with label Mohr. Show all posts
Wednesday, May 7, 2014
Monday, March 3, 2014
Methods paper on fly RNAi in cells and in vivo
Humbly offered by your blog author ...
Mohr SE. RNAi screening in Drosophila cells and in vivo. Methods. 2014 Feb 24. pii: S1046-2023(14)00062-0. PMID: 24576618.
Mohr SE. RNAi screening in Drosophila cells and in vivo. Methods. 2014 Feb 24. pii: S1046-2023(14)00062-0. PMID: 24576618.
Monday, July 29, 2013
DRSC announces launch of FlyPrimerBank online resource
The DRSC is pleased to announce the launch of FlyPrimerBank.
FlyPrimerBank is built around a database of three precomputed qPCR primer pairs per Drosophila gene. Online search output includes information about the primer pairs and when available, validation test results from our group or the community at large.
For details please see our paper, just out in the journal G3. You can also check out the documentation page.
Important for RNAi studies, FlyPrimerBank can report overlap between the primers and RNAi reagents in public collections. This helps you avoid choosing primers that could amplify the reagent.
As always, your feedback on the online resource is welcome.
FlyPrimerBank also makes it possible for you to indicate which qPCR primers in FlyPrimerBank have or have not worked for them, or to upload the sequences of good primer pairs not already in the database.
With your help, the utility of the resource will further increase over time.
FlyPrimerBank is built around a database of three precomputed qPCR primer pairs per Drosophila gene. Online search output includes information about the primer pairs and when available, validation test results from our group or the community at large.
For details please see our paper, just out in the journal G3. You can also check out the documentation page.
Important for RNAi studies, FlyPrimerBank can report overlap between the primers and RNAi reagents in public collections. This helps you avoid choosing primers that could amplify the reagent.
As always, your feedback on the online resource is welcome.
FlyPrimerBank also makes it possible for you to indicate which qPCR primers in FlyPrimerBank have or have not worked for them, or to upload the sequences of good primer pairs not already in the database.
With your help, the utility of the resource will further increase over time.
Wednesday, June 26, 2013
UP-TORR. A new tool from the DRSC. Find RNAi reagents--using today's information!
The DRSC has launched a new tool!
And a corresponding paper is now out in the journal Genetics.
Hu Y, Roesel C, Flockhart I, Perkins L, Perrimon N, Mohr S. UP-TORR: Online Tool for Accurate and Up-to-Date Annotation of RNAi Reagents. Genetics. 2013 Jun 21. PMID: 23792952.
UP-TORR, named for "Updated Targets of RNAi Reagents" checks daily for new updates to fly gene annotations and RNAi reagent collections (including TRiP, NIG-Japan and VDRC RNAi fly stocks). This lets you view the most updated interpretation of the gene-to-RNAi-reagent relationship.
Why does this matter? Because gene annotations change--sometimes a lot--over time, changing the interpretation of on-targets and off-targets of a reagent.
Please check out our paper to learn more. We also have a help page and a video demo online. As always, feedback on the online tool is welcome.
And a corresponding paper is now out in the journal Genetics.
Hu Y, Roesel C, Flockhart I, Perkins L, Perrimon N, Mohr S. UP-TORR: Online Tool for Accurate and Up-to-Date Annotation of RNAi Reagents. Genetics. 2013 Jun 21. PMID: 23792952.
UP-TORR, named for "Updated Targets of RNAi Reagents" checks daily for new updates to fly gene annotations and RNAi reagent collections (including TRiP, NIG-Japan and VDRC RNAi fly stocks). This lets you view the most updated interpretation of the gene-to-RNAi-reagent relationship.
Why does this matter? Because gene annotations change--sometimes a lot--over time, changing the interpretation of on-targets and off-targets of a reagent.
Please check out our paper to learn more. We also have a help page and a video demo online. As always, feedback on the online tool is welcome.
Wednesday, November 16, 2011
DRSC Database Paper
Our database update paper is published. As always, feedback on the database and website is welcome. I'm hoping to catch up with some breaking research reports soon.
Flockhart et al. (2011) FlyRNAi.org--the database of the Drosophila RNAi screening center: 2012 update. Nucleic Acids Res. PubMed ID: 22067456.
Flockhart et al. (2011) FlyRNAi.org--the database of the Drosophila RNAi screening center: 2012 update. Nucleic Acids Res. PubMed ID: 22067456.
Wednesday, October 5, 2011
Breaking Report: Review of RNAi screening
Norbert and I have a new review out that discusses cell-based and in vivo RNAi in various species and emphasizes interaction of the RNAi screening field with other fields of study.
Mohr & Perrimon (2011) RNAi screening: new approaches, understandings, and organisms. Wiley Interdiscip Rev RNA. doi: 10.1002/wrna. PubMed ID: 21953743
Mohr & Perrimon (2011) RNAi screening: new approaches, understandings, and organisms. Wiley Interdiscip Rev RNA. doi: 10.1002/wrna. PubMed ID: 21953743
Wednesday, August 31, 2011
DRSC's DIOPT and DIOPT-DIST
Sneak peak for blog readers: Our new ortholog-related tools DIOPT and DIOPT-DIST are available online and a provisional version of the paper describing the tools is also available at BMC Bioinformatics. As always, your feedback on our web-based tools is welcome!
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