Breaking Report: Cross-Species Transgenesis

Also on my desk today--a pair of papers on techniques relevant to cross-species RNAi rescue.

Ejsmont et al. (2011) Recombination-mediated genetic engineering of large genomic DNA transgenes. Methods Mol Biol. 772:445-58. PubMed ID: 22065454.

Ejsmont et al. (2011) Production of fosmid genomic libraries optimized for liquid culture recombineering and cross-species transgenesis. Methods Mol Biol. 772:423-43. PubMed ID: 22065453.

Breaking Report: Ecdysone Receptor Co-Factors

Also on my desk today? Catching up on a new small-scale fly cell RNAi screen. The authors tested one dsRNA per gene with several different cell lines in this focused study.

Davis et al. (2011) Identification of common and cell type specific LXXLL motif EcR cofactors using a bioinformatics refined candidate RNAi screen in Drosophila melanogaster cell lines. BMC Dev Biol. 11(1):66. PubMed ID: 22050674.

Reminder: The DRSC can help with small-scale screens by building custom libraries in 96- or 384-well plates based on our existing libraries of templates.

Breaking Report: Stable Cell Lines

On my desk today? A new report on making stable cell lines.

Gonzalez et al. (2011) Generation of stable Drosophila cell lines using multicistronic vectors. In Nature's Scientific Reports. DOI: 10.1038/srep00075

DRSC Database Paper

Our database update paper is published. As always, feedback on the database and website is welcome. I'm hoping to catch up with some breaking research reports soon.

Flockhart et al. (2011) FlyRNAi.org--the database of the Drosophila RNAi screening center: 2012 update. Nucleic Acids Res. PubMed ID: 22067456.