On my desk to ready today? A thought-provoking review on progress made and the future of RNAi as a tool in mammalian cells.
Kaelin WG Jr. Molecular biology. Use and abuse of RNAi to study mammalian gene function. Science. 2012 Jul 27;337(6093):421-2. PubMed PMID: 22837515.
Among the recommendations made for the future of mammalian RNAi is screening of smaller focused gene sets with more unique reagents per gene. The DRSC facilitates a similar approach in Drosophila cell-based RNAi screening via availability of deeper-coverage focused or 'sub' libraries and custom dsRNA synthesis for a set of genes based on our existing collection of templates.