Tuesday, April 22, 2014

New report on using CRISPR/Cas9 in Drosophila cells

Böttcher R, Hollmann M, Merk K, Nitschko V, Obermaier C, Philippou-Massier J, Wieland I, Gaul U, Förstemann K. Efficient chromosomal gene modification with CRISPR/cas9 and PCR-based homologous recombination donors in cultured Drosophila cells. Nucleic Acids Res. 2014 Apr 19. PMID: 24748663.

From the abstract:  "We present a series of PCR template vectors for C-terminal protein tagging and clonal Drosophila S2 cell lines with stable expression of a myc-tagged cas9 protein." The authors also state that the plasmid constructs associated with the publication will be available at AddGene.

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