Tuesday, August 26, 2014

Fly CRISPR -- fluorescent tag and RMCE approach

Learned about this pre-print online from the Insect Genetic Technologies Research Coordination Network (see "technology tips" section).

Describes use of fluorescent tagging to more efficiently identify successful knock-ins, then RMCE to change the knocked-in cassette.

Xu Zhang, Wouter Koolhaas, Frank Schnorrer. A versatile two-step CRISPR- and RMCE-based strategy for efficient genome engineering in Drosophila. doi: http://dx.doi.org/10.1101/007864

Pre-print is available online:

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