Thursday, October 30, 2014

Full-genome screen related to functional insulators

Another full-genome DRSC screen published--congrats!

Fig. 1 from the Bohla et al. paper.
Bohla D, Herold M, Panzer I, Buxa MK, Ali T, Demmers J, Kr├╝ger M, Scharfe M, Jarek M, Bartkuhn M, Renkawitz R. A Functional Insulator Screen Identifies NURF and dREAM Components to Be Required for Enhancer-Blocking. PLoS One. 2014 Sep 23;9(9):e107765. PubMed PMID: 25247414; PubMed Central PMCID: PMC4172637.

From the abstract: "... Here, we wanted to identify chromatin modification and remodelling factors required for an enhancer blocking function. We used the well-studied Fab-8 insulator of the bithorax locus to apply a genome-wide RNAi screen for factors that contribute to the enhancer blocking function of CTCF and CP190. Among 78 genes required for optimal Fab-8 mediated enhancer blocking, all four components of the NURF complex as well as several subunits of the dREAM complex were most evident. ..."

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