Thursday, August 27, 2015

New resource--Enhancer trap flippase fly stocks

Smith BN, Ghazanfari AM, Bohm RA, Welch WP, Zhang B, Masly JP. A Flippase-Mediated GAL80/GAL4 Intersectional Resource for Dissecting Appendage Development in Drosophila. G3 (Bethesda). 2015 Aug 13. pii: g3.115.019810. PMID: 26276385.

From the abstract: "... A recently developed Flippase-induced intersectional GAL80/GAL4 repression method incorporates several gene manipulation technologies in Drosophila to enable such fine-scale dissection in neural tissues. ... Here, we expand the utility of a large collection of these enhancer-trap flippase transgenic insertion lines by characterizing their expression patterns in third larval instar imaginal discs. We screened 521 different enhancer-trap flippase lines and identified 28 that are expressed in imaginal tissues, including two transgenes that show sex-specific expression patterns. ... The results of our experiments show that these enhancer-trap flippase lines enable fine-scale manipulation in imaginal discs."

No comments: