Lucy Cherbas, Jennifer Hackney, Lei Gong, Claire Salzer, Eric Mauser, Dayu Zhang and Peter Cherbas. 2015. Tools for Targeted Genome Engineering of Established Drosophila Cell Lines. Early online at Genetics.
From the abstract: "We describe an adaptation of φC31 integrase-mediated targeted cassette exchange for use in Drosophila
cell lines. ... We demonstrated the technology by integrating a
cassette
containing a Cu++-inducible mCherry
marker, and we report the expression properties of those lines. When
compared with clonal lines made by
traditional transformation methods, which lead to
the illegitimate insertion of tandem arrays, targeted insertion lines
give
more uniform expression, lower basal expression and
higher induction ratios. Targeted substitution, though intricate,
affords
results that should greatly improve comparative
expression assays – a major emphasis of cell-based studies."
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