Gou B, Zhu E, He R, Stern U, Yang CH. High Throughput Assay to Examine Egg-Laying Preferences of Individual Drosophila melanogaster. J Vis Exp. 2016 Mar 24;(109). PMID: 27077482.
From the abstract: "Recently, egg-laying preference of Drosophila has emerged as a genetically tractable model to study the neural basis of simple decision-making processes. ... To increase the throughput of studying of egg-laying preferences of single females, we developed custom chambers that each can simultaneously assay egg-laying preferences of up to thirty individual flies as well as a protocol that ensures each female has a high egg-laying rate ... This article provides the blueprints for fabricating these chambers and the procedure for preparing the flies to be assayed in these chambers."