Friday, June 10, 2016

Combined CRISPR-RNAi approach

On my desk to read today? This report from former Perrimonian and DRSC screener R. Neumuller:

Wissel S, Kieser A, Yasugi T, Duchek P, Roitinger E, Gokcezade JF, Steinmann V, Gaul U, Mechtler K, F├Ârstemann K, Knoblich JA, Neum├╝ller RA. A Combination of CRISPR/Cas9 and Standardized RNAi as a Versatile Platform for the Characterization of Gene Function. G3 (Bethesda). 2016 Jun 8. pii: g3.116.028571. PMID: 27280787.

From the abstract: "... miGFPi combines CRISPR/Cas9 mediated tagging of genes at their endogenous locus with an immunotag and an exogenous 21 nucleotide RNAi effector sequence with the use of a single reagent, highly validated RNAi line targeting this sequence. We demonstrate the utility and time effectiveness of this method ... In addition, we show that miGFPi serves as a powerful technique to functionally characterize individual isoforms of a gene. ..."

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