Thursday, May 31, 2018

New in vivo RNAi screen report -- larval chordotonal organ morphogenesis

An RNAi Screen Identifies New Genes Required for Normal Morphogenesis of Larval Chordotonal Organs

Abeer Hassan, Yael Timerman, Rana Hamdan, Nitzan Sela,  Adel Avetisyan,  Naomi Halachmi and Adi Salzberg
G3: GENES, GENOMES, GENETICS June 1, 2018 vol. 8 no. 6 1871-1884

From the abstract: "... Here we describe an RNAi screen that focused on the ChO’s accessory and attachment cells and was performed in 2nd instar larvae to allow for phenotypic analysis of ChOs that had already experienced mechanical stresses during larval growth. Nearly one thousand strains carrying RNAi constructs targeting more than 500 candidate genes were screened for their effects on ChO morphogenesis. The screen identified 31 candidate genes whose knockdown within the ChO lineage disrupted various aspects of cell fate determination, cell differentiation, cellular morphogenesis and cell-cell attachment. ..."

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