These two papers, one of which is co-authored by folks here at the DRSC, describe using germline expression of Cas9 for CRISPR genome engineering approaches.
Sebo ZL, Lee HB, Peng Y, Guo Y. A simplified and efficient germline-specific CRISPR/Cas9 system for Drosophila genomic engineering. Fly (Austin). 2013 Oct 18;8(1). PMID: 24141137.
Ren X, Sun J, Housden BE, Hu Y, Roesel C, Lin S, Liu LP, Yang Z, Mao D, Sun L, Wu Q, Ji JY, Xi J, Mohr SE, Xu J, Perrimon N, Ni JQ. Optimized gene editing technology for Drosophila melanogaster using germ line-specific Cas9. Proc Natl Acad Sci U S A. 2013 Nov 4. PMID: 24191015. Note: This paper includes a description of our searchable online resource of pre-computed CRISPR sgRNA designs.
Showing posts with label Sebo. Show all posts
Showing posts with label Sebo. Show all posts
Wednesday, November 6, 2013
Tuesday, October 29, 2013
More new reports on fly genome engineering.
Sebo ZL, Lee HB, Peng Y, Guo Y. A simplified and efficient germline-specific
CRISPR/Cas9 system for Drosophila genomic engineering. Fly (Austin). 2013 Oct
18;8(1). PMID: 24141137.
Gratz SJ, Wildonger J, Harrison MM, O'Connor-Giles KM. CRISPR/Cas9-mediated genome engineering and the promise of designer flies on demand. Fly (Austin). 2013 Oct 2;7(4). PMID: 24088745.
Baena-Lopez LA, Alexandre C, Mitchell A, Pasakarnis L, Vincent JP. Accelerated homologous recombination and subsequent genome modification in Drosophila. Development. 2013 Oct 23. PMID: 24154526.
Click here to view all posts tagged CRISPRs.
Gratz SJ, Wildonger J, Harrison MM, O'Connor-Giles KM. CRISPR/Cas9-mediated genome engineering and the promise of designer flies on demand. Fly (Austin). 2013 Oct 2;7(4). PMID: 24088745.
Baena-Lopez LA, Alexandre C, Mitchell A, Pasakarnis L, Vincent JP. Accelerated homologous recombination and subsequent genome modification in Drosophila. Development. 2013 Oct 23. PMID: 24154526.
Click here to view all posts tagged CRISPRs.
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