On my desk today? A paper reporting the results of doing the same screen in several Drosophila cell types.
Liu, Sims & Baum (2009) A parallel RNAi screen across different cell lines identifies generic and cell-type specific regulators of actin organisation and cell morphology. Genome Biology 10:R26.
Protocol Notes: They designed and built a kinome RNAi library based on gene-specific primers in their FLIGHT db or using the DFKZ E-RNAi tool. Kc167, S2R+, S2, BG2-c2, BG3-c2 and BG3-c1 lines tested. Microtubule, actin and DNA staining read-outs with imaging.
Reminds me of: Some papers that describe the results of cross cell-type screens in mammalian cells. I am aware of at least two such reports (please comment if you know of others).
Grueneberg et al. (2008) Kinase requirements in human cells: I. Comparing kinase requirements across various cell types. PNAS 105(43):16472-7.
Luo et al. (2008) Highly parallel identification of essential genes in cancer cells. 105(51):20380-5.
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