Today I'm thinking most about transfection. As with any protocol, I'm interested in two things. How to do it well. And how to do it well cheaply--particularly important when you're doing things at high-throughput scale as researchers do here for whole-genome screens.
Based on experience, we've been using Qiagen Effectene as a transfection reagent. Some visiting screeners use a reagent from Invitrogen. And I recently got a protocol from the folks at Genesee Scientific for FectoFly (made in Europe by PolyPlus).
Have feedback? Experience? Favorites? We are interested to hear about it. So we can provide the best advice on the DRSC website and to visiting researchers.
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