Monday, February 23, 2015

Sleep screens--methods review includes discussion of in vivo RNAi

Axelrod S, Saez L, Young MW. Studying circadian rhythm and sleep using genetic screens in Drosophila. Methods Enzymol. 2015;551:3-27. PMID: 25662449.

From the abstract: "... In this chapter, we briefly recall the history of circadian rhythm and sleep screens and then ... describe techniques ... for ... screening in the field.  ... comparing the newer approaches of transgenic RNA interference (RNAi) to classical forms of mutagenesis ... We discuss the different screening approaches in light of the literature and published and unpublished sleep and rhythm screens utilizing ethyl methanesulfonate mutagenesis and transgenic RNAi from our lab."

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