Tuesday, June 2, 2015

Actin in the nucleus -- genome-wide cell-based RNAi screen

Excited to see this report on a screen performed by J. Dopie at the DRSC!

Dopie J, Rajakylä EK, Joensuu MS, Huet G, Ferrantelli E, Xie T, Jäälinoja H, Jokitalo E, Vartiainen MK. Genome-wide RNAi screen for nuclear actin reveals a network of cofilin regulators. J Cell Sci. 2015 May 28. pii: jcs.169441. PMID: 26021350.

From the abstract: "Nuclear actin plays an important role in many processes that regulate gene expression. ... Here we have performed a genome-wide RNAi screen in Drosophila cells to identify proteins that influence either nuclear polymerization or import of actin. We validate 19 factors as specific hits, and show that Chinmo/Bach2, SNF4Aγ/Prkag1 and Rab18 play a role in nuclear localization of actin in both fly and mammalian cells. We identify several novel regulators of cofilin activity, and characterize modulators of both cofilin kinases and phosphatase. ... Our screen therefore reveals novel aspects of actin regulation and links nuclear actin to many cellular processes."

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