Breaking Report: A Look at Centromeres

What am I reading today? Another paper that reports results from a genome-wide study performed at the DRSC: Erhardt et al. (2008) Genome-wide analysis reveals a cell cycle-dependent mechanism controlling centromere propagation. JCB 183(5):805-818.

The authors describe the results of a full-genome screen for factors that disrupt the normal localization of CENP-A (CID). Protocol Notes: A fluorescence image-based screen assay in Kc167 cells. The authors provide a nicely detailed description of the screening protocol, including info on image analysis, in the Materials & Methods section. Follow-up includes time-lapse and additional imaging in S2 cells and fly embryos. Plus IP from Kc167 cells.