Wednesday, April 15, 2009

Breaking Report: single-cell RNA-Seq

On my desk today? Tang et al. (2009) mRNA-Seq whole-transcriptome analysis of a
single cell in Nature Methods.

Why this paper? At the protocol level, it's good to see what's resulting from availability of next generation sequencing technologies. And at the biological level, interesting to note that the authors found that about 1500 genes were up-regulated in Dicer1 -/- or Ago2 -/- mouse oocytes (with about 600 up-regulated in both genetic backgrounds).

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