More new reports on fly genome engineering.

Sebo ZL, Lee HB, Peng Y, Guo Y. A simplified and efficient germline-specific CRISPR/Cas9 system for Drosophila genomic engineering. Fly (Austin). 2013 Oct 18;8(1). PMID: 24141137.

Gratz SJ, Wildonger J, Harrison MM, O'Connor-Giles KM. CRISPR/Cas9-mediated genome engineering and the promise of designer flies on demand. Fly (Austin). 2013 Oct 2;7(4). PMID: 24088745.

Baena-Lopez LA, Alexandre C, Mitchell A, Pasakarnis L, Vincent JP. Accelerated homologous recombination and subsequent genome modification in Drosophila. Development. 2013 Oct 23. PMID: 24154526.

Click here to view all posts tagged CRISPRs.

DRSC DIOPT and DIOPT-DIST ortholog tools now include Xenopus and S. pombe

The DRSC is pleased to announce that our DIOPT and DIOPT-DIST tools for ortholog search now include Xenopus and S. pombe in addition to fly, worm, S. cerevisiae, zebrafish, mouse and human. We added these two new species in response to community feedback.

What's the difference between DIOPT and DIOPT-DIST?

DIOPT lets you search for orthologs for any pair of species (e.g. zebrafish orthologs of fly genes, or yeast orthologs of a mouse gene). Results from 10 different public tools/alorithms are shown.

DIOPT-DIST lets you search using any of the non-human species we report, and view not just putative human orthologs but also connections between those human orthologs and disease. Alternatively, you can start with a disease, view human genes associated with that disease and orthologs of those human genes in a given species of interest.

If you use these tools, please cite our paper:  Hu et al. BMC Bioinformatics. PMID: 21880147

DRSC online database of pre-computer CRISPR sgRNAs

The DRSC is pleased to announce the launch of a searchable database of pre-computed CRISPR sgRNA sequences targeting the fly genome. As always, your feedback is welcome.

GenomeRNAi launches 11.1 and asks for feedback

The folks at the DKFZ's GenomeRNAi have launched a version 11.1 and ask for feedback, in particular on the beta version of their 'screen comparison' tool.

in vivo screen of GPCRs that regulate flight. Recent report.

Agrawal T, Sadaf S, Hasan G. A Genetic RNAi Screen for IP(3)/Ca(2+) Coupled GPCRs in Drosophila Identifies the PdfR as a Regulator of Insect Flight. PLoS Genet. 2013 Oct;9(10):e1003849. PMID: 24098151; PMCID: PMC3789835.

 From the abstract: "... RNAi screen to identify GPCRs that regulate flight by activating the IP3 receptor ..."

Validation with RNAi in study of the Hippo pathway interactome. Recent report.

Kwon Y, Vinayagam A, Sun X, Dephoure N, Gygi SP, Hong P, Perrimon N. The Hippo Signaling Pathway Interactome. Science. 2013 Oct 10. PMID: 24114784.

From the abstract: "... a high-confidence Drosophila Hippo protein-protein interaction network (Hippo-PPIN) consisting of 153 proteins and 204 interactions. Depletion of 67% of the proteins by RNAi regulated the transcriptional coactivator Yorkie (Yki) either positively or negatively."

Validation with RNAi in study of sensory neurons. Recent report.

Iyer EP, Iyer SC, Sullivan L, Wang D, Meduri R, Graybeal LL, Cox DN. Functional genomic analyses of two morphologically distinct classes of Drosophila sensory neurons: post-mitotic roles of transcription factors in dendritic patterning. PLoS One. 2013 PMID: 23977298; PMCID: PMC3744488.

From the abstract: "We have used a combination of cell-type specific transcriptional expression profiling coupled to a targeted and systematic in vivo RNAi functional validation screen."