Monday, June 16, 2014

Protocol report on transgenic ORF strains

Bischof J, Sheils EM, Björklund M, Basler K. Generation of a transgenic ORFeome library in Drosophila. Nat Protoc. 2014 Jul;9(7):1607-20. PMID: 24922270.

From the abstract: "Here we provide a protocol for high-throughput cloning of Drosophila open-reading frames (ORFs) that are regulated by upstream activation sequences (UAS sites) ... We also provide details for FLP/FRT-mediated in vivo exchange of epitope tags (or regulatory regions) in the ORF library strains, which further extends the potential applications of the library. These transgenic UAS-ORF strains are a useful resource to complement and validate genetic experiments performed with loss-of-function mutants and RNA interference (RNAi) lines."

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