Over-expression of transcription factors--resource and screen paper from Deplancke lab

Early online at Genome Research:  A large-scale, in vivo transcription factor screen defines bivalent chromatin as a key property of regulatory factors mediating Drosophila wing development. Claus Schertel, Monica Albarca, Claudia Rockel-Bauer, Nicholas W Kelley, Johannes Bischof, Korneel Hens, Erik van Nimwegen, Konrad Basler and Bart Deplancke. 2015.

From the abstract: "... We generated 596 site-directed transgenic Drosophila lines that contain integrations of individual UAS-TF constructs to facilitate spatio-temporally controlled misexpression in vivo. All transgenes were expressed in the developing wing and two thirds induced specific phenotypic defects. In vivo knock-down of the same genes yielded a phenotype for 50%, with both methods indicating a great potential for misexpression to characterize novel functions in wing growth, patterning and development. Thus, our UAS-TF library provides an important addition to the genetic toolbox of Drosophila research ..."

Protocol report on transgenic ORF strains

Bischof J, Sheils EM, Björklund M, Basler K. Generation of a transgenic ORFeome library in Drosophila. Nat Protoc. 2014 Jul;9(7):1607-20. PMID: 24922270.

From the abstract: "Here we provide a protocol for high-throughput cloning of Drosophila open-reading frames (ORFs) that are regulated by upstream activation sequences (UAS sites) ... We also provide details for FLP/FRT-mediated in vivo exchange of epitope tags (or regulatory regions) in the ORF library strains, which further extends the potential applications of the library. These transgenic UAS-ORF strains are a useful resource to complement and validate genetic experiments performed with loss-of-function mutants and RNA interference (RNAi) lines."