Bischof J, Sheils EM, Björklund M, Basler K. Generation of a transgenic ORFeome library in Drosophila. Nat Protoc. 2014 Jul;9(7):1607-20. PMID: 24922270.
From the abstract: "Here we provide a protocol for high-throughput cloning of Drosophila open-reading frames (ORFs) that are regulated by upstream activation sequences (UAS sites) ... We also provide details for FLP/FRT-mediated in vivo exchange of epitope tags (or regulatory regions) in the ORF library strains, which further extends the potential applications of the library. These transgenic UAS-ORF strains are a useful resource to complement and validate genetic experiments performed with loss-of-function mutants and RNA interference (RNAi) lines."
Showing posts with label Basler. Show all posts
Showing posts with label Basler. Show all posts
Monday, June 16, 2014
Monday, September 5, 2011
Breaking Report: Full-Genome Screen for Wg Secretion Regulators
On my desk to read today?
A report on a full-genome cell-based RNAi screen performed here at the DRSC.
Port et al. (2011) A genome-wide RNA interference screen uncovers two p24 proteins as regulators of Wingless secretion. EMBO Rep. doi: 10.1038/embor.2011.165. PMID: 21886182.
A report on a full-genome cell-based RNAi screen performed here at the DRSC.
Port et al. (2011) A genome-wide RNA interference screen uncovers two p24 proteins as regulators of Wingless secretion. EMBO Rep. doi: 10.1038/embor.2011.165. PMID: 21886182.
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