Wednesday, March 18, 2015

CRISPR off-targets study in human cells

Findings relevant to fly studies? Will be interesting to see.

Kim D, Bae S, Park J, Kim E, Kim S, Yu HR, Hwang J, Kim JI, Kim JS. Digenome-seq: genome-wide profiling of CRISPR-Cas9 off-target effects in human cells. Nat Methods. 2015 Mar;12(3):237-43. PMID: 25664545.

From the abstract: "... genome-wide target specificities of Cas9 nucleases remain controversial. Here we present Digenome-seq, in vitro Cas9-digested whole-genome sequencing, to profile genome-wide Cas9 off-target effects in human cells. ... We also showed that Cas9 nucleases can be highly specific ... and that Cas9 off-target effects can be avoided by replacing 'promiscuous' single guide RNAs (sgRNAs) with modified sgRNAs. ..."

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