Wednesday, March 25, 2015

"Trojan exon" variation on the theme of MiMIC and CRISPR insertion strategies

Diao F, Ironfield H, Luan H, Diao F, Shropshire WC, Ewer J, Marr E, Potter CJ, Landgraf M, White BH. Plug-and-Play Genetic Access to Drosophila Cell Types using Exchangeable Exon Cassettes. Cell Rep. 2015 Mar 3;10(8):1410-21. PMID: 25732830.

From the abstract: "... we introduce a simple, versatile method for achieving cell-type-specific expression of transgenes ... Our method couples the expression of a transgene to that of a native gene expressed in the cells of interest ... We demonstrate the efficacy of this approach in Drosophila ... We also introduce an exchangeable, MiMIC-like Trojan exon construct that can be targeted to coding introns using the Crispr/Cas system."

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