Monday, March 30, 2015

Report of 'systems-level interrogation' includes DRSC RNAi screen data, phosphoproteomics and in vivo analyses

Sopko R, Lin YB, Makhijani K, Alexander B, Perrimon N, Brückner K. A systems-level interrogation identifies regulators of Drosophila blood cell number and survival. PLoS Genet. 2015 Mar 6;11(3):e1005056. PMID: 25749252; PMCID: PMC4352040.

From the abstract: "Here, we study signaling by the Drosophila PDGF/VEGF Receptor (Pvr) in embryonic blood cells (hemocytes) and in the related cell line Kc as a model for the requirement of PDGF/VEGF receptors in vertebrate cell survival and proliferation. ... Using Kc cells, we performed a genome wide RNAi screen for regulators of cell number in a sensitized, Pvr deficient background. We identified the receptor tyrosine kinase (RTK) Insulin-like receptor (InR) as a major Pvr Enhancer, and ... Ecdysone receptor (EcR) and ultraspiracle (usp) ... as Pvr Suppressors. ... Phosphoproteomic analysis demonstrates distinct modes of cell number regulation by EcR and RTK signaling. ... our analysis reveals that the selection of phosphorylation targets by signaling receptors shows qualitative changes depending on the signaling status of the cell, which may have wide-reaching implications for other cell regulatory systems."

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