Denton D, Kumar S. Analyzing the Response of RNAi-Treated Drosophila Cells to Death Stimuli by Quantitative Real-Time Polymerase Chain Reaction. Cold Spring Harb Protoc. 2015 Jul 1;2015(7):pdb.prot086223. PMID: 26134907.
From the abstract: "A useful complement to animal studies is the use of Drosophila cell lines to analyze cell-death responses. ... Drosophila cell lines are very amenable to knockdown studies ... the cell lines are useful for investigating the response to death stimuli, following gene knockdown, by examining the expression of cell-death genes. This protocol describes the synthesis of dsRNA for treatment of Drosophila cells and the subsequent analysis of cell-death gene expression by quantitative real-time polymerase chain reaction (qPCR)."
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