Monday, July 20, 2015

S2 cell-based assay related to bioactive lipids

Wang P, Wang Q, Yang L, Qin QL, Wu YJ. Characterization of lysophosphatidylcholine-induced changes of intracellular calcium in Drosophila S2 cells. Life Sci. 2015 Jun 15;131:57-62. PMID: 25736969.

From the abstract: "Lysophosphatidylcholine (LPC), a bioactive lipid, regulates a wide array of biological processes. ... We aimed to study the mechanism of LPC-induced [Ca(2+)]i changes in Drosophila S2 cells. ... The [Ca(2+)]i of Drosophila S2 cells was measured by fluorescence spectrophotometry after loading the cells with the calcium-sensitive fluorescent probe Fura-2/AM. ...
Our results demonstrated that LPC could cause a rapid, dose-dependent increase in the [Ca(2+)]i in the presence of external calcium ([Ca(2+)]e). ... The LPC-induced [Ca(2+)]i increase in S2 cells characterized in this study may shed light on the study of NTE/SWS protein function in general because the enzyme is responsible for the deacylation of LPC."

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