Wang P, Wang Q, Yang L, Qin QL, Wu YJ. Characterization of lysophosphatidylcholine-induced changes of intracellular calcium in Drosophila S2 cells. Life Sci. 2015 Jun 15;131:57-62. PMID: 25736969.
From the abstract: "Lysophosphatidylcholine
(LPC), a bioactive lipid, regulates a wide array of biological
processes. ... We aimed to study the mechanism of
LPC-induced [Ca(2+)]i changes in Drosophila S2 cells. ... The
[Ca(2+)]i of Drosophila S2 cells was measured by fluorescence
spectrophotometry after loading the cells with the calcium-sensitive
fluorescent probe Fura-2/AM. ...
Our
results demonstrated that LPC could cause a rapid, dose-dependent
increase in the [Ca(2+)]i in the presence of external calcium
([Ca(2+)]e). ... The LPC-induced [Ca(2+)]i increase in S2 cells characterized in
this study may shed light on the study of NTE/SWS protein function in
general because the enzyme is responsible for the deacylation of LPC."
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