Yao C, Sasaki HM, Ueda T, Tomari Y, Tadakuma H. Single-Molecule Analysis of the Target Cleavage Reaction by the Drosophila RNAi Enzyme Complex. Mol Cell. 2015 Jul 2;59(1):125-32. PMID: 26140368.
From the abstract: "Small interfering RNAs (siRNAs) direct cleavage of complementary target
RNAs via an RNA-induced silencing complex (RISC) that contains
Argonatute2 protein at its core. However, what happens after target
cleavage remains unclear. Here we analyzed the cleavage reaction by
Drosophila Argonaute2-RISC using single-molecule imaging and revealed a
series of intermediate states in target recognition, cleavage, and
product release. Our data suggest that, after cleavage, RISC generally
releases the 5' cleavage fragment from the guide 3' supplementary region
first and then the 3' fragment from the seed region, highlighting the
reinforcement of the seed pairing in RISC. ... "